Vitantonio Pantaleo

At present my interests focus on RNA silencing-based plant-pathogen interaction mechanisms with a particular attentions to the post-transcriptional gene silencing. My studies implicate the use of both classic cell/molecular biology and Next Generation Sequences approaches. The aim is to add some layer of knowledge in this field through the identification and characterization of functional short non-coding RNAs and their targets of both endogenous (plant genome) or exogenous (pathogen) origin. Diversity and functions of plant effector proteins involved in the RNA silencing pathways are also targets of my studies (i.e. Argonautes, AGOs; Dicers, DCLs; RNA polimerases, RDR).

The genome of positive strand RNA plant viruses i) is recognized as a foreigner by the host plant, ii) it induces RNA silencing and iii) it contains all the signals to use make advantage of the host translational machinery to be translated, similarly to host mRNAs. Therefore my research is carried out mainly using the model system Nicotiana benthamiana (or other solanaceous plants) – virus (Tombusvirus or other positive strand RNA viruses). In collaboration with other Italian and European research groups other in vitro (cell lysates) and in vivo heterologous systems (Saccharomyces cerevisiae) are also used in support of my researches.

Specific founded research projects are allowing to transfer some of the acquired results and knowledge to crop system with relevant impact i.e. Vitis vinifera, Solanum lycopericum e Cicer arietinum in order to pursue applicative objectives such as viral metagenomics in plant populations, mechanism plant resilience to biotic-abiotic stresses, conservation and charcterization of plant diversity.

Projects

Articles and book chapters since 2008

  • 2018 - A Short Indel-Lacking-Resistance Gene Triggers Silencing of the Photosynthetic Machinery Components Through TYLCSV-Associated Endogenous siRNAs in Tomato - Chiumenti M., Catacchio C.R., Miozzi L., Pirovano W., Ventura M., Pantaleo V. - Frontiers in plant science , 9: 1470
  • 2018 - Shannon entropy to evaluate substitution rate variation among viral nucleotide positions in datasets of viral siRNAs - Ghasemzadeh A., Shams-Bakhsh M., Pirovano W., Pantaleo V. - Methods In Molecular Biology , 1746: 187-195
  • 2018 - Viral Metagenomics - Pantaleo V., Chiumenti M. (eds.) - in: Viral Metagenomics. Methods And Protocols ,
  • 2018 - Viruses and phytoparasitic nematodes of Cicer arietinum L.: biotechnological approaches in interaction studies and for sustainable control - Leonetti, P., Accotto, G.P., Hanafy, M. S., Pantaleo V. - Frontiers In Plant Science , volume 9
  • 2018 - A viral suppressor modulates the plant immune response early in infection by regulating miRNA activity - Pertermann R, Tamilarasan S., Gursinsky T., Gambino G., Schuck J., Weinholdt C., Lilie H., Grosse I., Golbik R., Pantaleo V. Behrens SE. - Mbio , volume 9
  • 2017 - Epigenetics in Plant-Pathogen Interactions - Gambino G., Pantaleo V. - Publisher: Springer International Publishing, 385-404
  • 2016 - Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress - Pantaleo V., Vitali M., Boccacci P., Miozzi L., Cuozzo D., Chitarra W., Mannini F., Lovisolo C., Gambino G. - Scientific Reports (Nature Publishing Group), 6: 20167
  • 2015 - Drawing siRNAs of viral origin out from plant siRNAs libraries - Miozzi L., Pantaleo V. - Methods in Molecular Biology, 1236:111-123
  • 2015 - Homeologs of the Nicotiana benthamiana antiviral ARGONAUTE1 show different susceptibilities to microRNA168-mediated control - Gursinsk T., Pirovano W., Gambino G., Friedrich S., Behrens S.E., Pantaleo V. - Plant Physiology (Online), 168:938-952
  • 2014 - Bioinformatics approaches for viral metagenomics in plants using short RNAs: model case of study and application to a Cicer arietinum population - Pirovano W., Miozzi L., Boetizer M., Pantaleo V. - Frontiers in Microbiology, 5:790
  • 2013 - Analysis of small RNAs derived from tomato yellow leaf curl Sardinia virus reveals a cross reaction between the major viral hotspot and the plant host genome - Miozzi L., Pantaleo V., Burgyán J., Accotto G.P., Noris E. - Virus Research, 178:287-296
  • 2013 - AGO/RISC-mediated antiviral RNA silencing in a plant in vitro system - Schuck J., Gursinsky T., Pantaleo V., Burgyan J., Behrens S.E. - Nucleic Acids Research, 41:5090-5103
  • 2013 - Piccoli RNA interferenti, immunità ai patogeni e applicazioni biotecnologiche - Pantaleo V., Barbarossa L. - Protezione delle colture, 1:11-19
  • 2013 - Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera - Miozzi L., Gambino G., Burgyan J., Pantaleo V. - Molecular Plant Pathology, 14:30-43
  • 2012 - Homology-independent discovery of replicating pathogenic circular RNAs by deep sequencing and a new computational algorithm - Wu Q., Wang Y., Cao M., Pantaleo V., Burgyan J., Li W.-X., Ding S.-W. - Proceedings of the National Academy of Sciences of The United States of America, 109:3938-3943
  • 2012 - Occurrence of Fig badnavirus 1 in Fig trees from different countries and in symptomless seedlings - Minafra A., Chiumenti M., Elbeaino T., Digiaro M., Bottalico G., Pantaleo V., Martelli G.P. - Journal of Plant Pathology, 94:S4.105
  • 2011 - Plant RNA silencing in viral defence - Pantaleo V. - Advances in Experimental Medicine and Biology, 722: 39-58.
  • 2011 - Viral induction and suppression of RNA silencing in plants - Shimura H, Pantaleo V. - Biochimica et Biophysica Acta, 1809: 601-612
  • 2011 - A Viral Satellite RNA Induces Yellow Symptoms on Tobacco by Targeting a Gene Involved in Chlorophyll Biosynthesis using the RNA Silencing Machinery - himura H., Pantaleo V., Ishihara T., Myojo N., Inaba J., Sueda K., Burgyán J., Masuta C. - Plos Pathogens, 7(5): e1002021
  • 2010 - Structural and functional analysis of viral siRNAs - Szittya G., Moxon S., Pantaleo V., Toth G., Pilcher R.L.R., Moulton V., Burgyan J., Dalmay T. - PLoS Pathogens, 6:1-15
  • 2010 - Deep sequencing analysis of viral short RNAs from an infected Pinot Noir grapevine - Pantaleo V., Saldarelli P., Miozzi L., Giampetruzzi A., Gisel A., Moxon S., Dalmay T., Bisztray G., Burgyan J. - Virology, 408:49-56
  • 2010 - Identification of grapevine microRNAs and their targets using high-throughput sequencing and degradome analysis - Pantaleo V., Szittya G., Moxon S., Miozzi L., Moulton V., Dalmay T., Burgyan J. - Plant Journal, 62:960-976
  • 2009 - RNA silencing: an antiviral mechanism - Csorba T., Pantaleo V., Burgyan J. - Advances in Virus Research, 75:35-71
  • 2009 - Deep sequencing of viroid-derived small RNAs from grapevine provides new insights on the role of RNA silencing in plant-viroid interaction - Navarro B., Pantaleo V., Gisel A., Moxon S., Dalmay T., Bisztray G., Di Serio F., Burgyan J. - PLoS ONE, 4:e7686
  • 2008 - Cymbidium ringspot virus harnesses RNA silencing to control the accumulation of virus parasite satellite RNA - Pantaleo V., Burgyan J. - Journal of Virology, 82:11851-11858
  • 2008 - Virus associated with fig mosaic in Hungary - De Stradis A., Pantaleo V., Salamon P., Minafra A., Martelli G.P. - Journal of Plant Pathology, 90:585