RESEARCH FELLOW
ceciliaparise(AT)cnr.it
publications: Orcid
personal details and research activity: People
Curriculum Vitae
In 2018, I obtained my bachelor’s degree in viticulture and oenology, and in 2022 I graduated in Plant health and sustainable cropping system (Erasmus Mundus Master).
To date, I am doing my PhD entitled: Exploring RNA interference (RNAi) on vector insects, mainly Philaenus spumarius. Thus, the main research activities carried out are focused on the application of RNAi in the spittlebug Philaenus spumarius, the main vector of Xylella fastidiosa, the causal agent of the olive tree rapid desiccation complex (OQDS). This allowed me to develop an excellent knowledge of the RNAi mechanism and vector-pathogen interactions. More in-depth tasks and skills acquired were in the knowledge and breeding of Philaenus spumarius, both in its juvenile and adult stages, also managing to manipulate its life cycle. With regard to laboratory activities, the main molecular techniques related to RNAi were included. Initially, target genes were chosen, their sequences were checked in vitro to avoid off-target effects, and double-stranded RNA (dsRNA) molecules were subsequently produced and tested separately on nymphs and adults of spittlebugs.
In addition, methods of dsRNA delivery to the insect were explored, in particular microinjection and acquisition by plant. Finally, molecular diagnostic techniques were learnt, namely RNA extraction, its retro-transcription to cDNA and evaluation of the silencing effect by qPCR. Diagnostics and prophylaxis of X. fastidiosa on the sputum and host plant were also studied.