Massimo Turina

Negli ultimi anni mi sono occupato di varie linee di ricerca, con un’impostazione virologica tradizionale, e con una nuova attenzione all’interazione ospite-patogeno, sia nel caso di virus vegetali, che nel caso di micovirus.

Nel dettaglio i principali argomenti di cui mi occupo sono:

  1. identificazione e caratterizzazione di nuove malattie ad eziologia virale, con particolare attenzione a nuove virosi di piante orticole e ornamentali.
  2. messa a punto di metodi diagnostici per virus e fitoplasmi, sia sierologici che molecolari
  3. interazione ospite-patogeno per ceppi di Tomato spotted wilt virus che superano la resistenza in pomodoro e peperone
  4. aspetti dell’interazione ospite-patogeno per Ourmia melon virus
  5. Interazione virus-vettore nel caso di TSWV e tripide Frankliniella occidentalis
  6. Alterazione delle cascate MAP chinasiche nel fungo ascomicete Cryphonectria parasitica infetto dal micovirus CHV1

Recentemente è stata intrapresa una nuova linea di ricerca in collaborazione con la Mycotheca Universitatis Taurinensis (MUT), che riguarda la ricerca di micovirus in una collezione di funghi isolati da Posidonia oceanica al fine di verificare le proprietà biologiche attribuibili alla presenza del micovirus stesso, in particolare riguardo ad un possibile vantaggio adattativo rispetto all’alta salinità dell’ambiente marino.

Progetti

Articoli in rivista e capitoli di libri dal 2008

  • 2018 - The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi - Turina M., Ghignone S., Astolfi N., Silvestri A., Bonfante P., Lanfranco L. - Environmental Microbiology ,
  • 2018 - Differenty approches to discover mycovirus associated to marine organism - Nerva L., Varese G.C., Turina M. - in: Viral Metagenomics. Methods And Protocols , pag. 97-114
  • 2017 - Mycoviruses of an endophytic fungus can replicate in plant cells: Evolutionary implications - Nerva L., Varese G.C., Falk B.W., Turina M. - Scientific Reports , volume 7
  • 2017 - ICTV virus taxonomy profile: Ourmiavirus - Turina M., Hillman B.I., Izadpanah K., Rastgou M., Rosa C., Lefkowitz E.J., Davison A.J., Siddell S.G., Simmonds P., Adams M.J., Smith D.B., Orton R.J., Sanfacon H. - Journal Of General Virology , 98: 129-130
  • 2017 - Full-length genome sequence of the tospovirus melon severe mosaic virus - Ciuffo M., Nerva L., Turina M. - Archives Of Virology , 162: 1419-1422
  • 2017 - The Torradovirus-specific RNA2-ORF1 protein is necessary for plant systemic infection - Ferriol I., Vallino M., Ciuffo M., Nigg J.C., Zamora-Macorra E.J., Falk B.W, Turina M. - Molecular Plant Pathology ,
  • 2017 - Genome sequence, prevalence and quantification of the first iflavirus identified in a phytoplasma insect vector - Abbà S., Galetto L., Vallino M., Rossi M., Turina M., Sicard A., Marzachì C. - Archives Of Virology , 162: 799–809
  • 2017 - Transmission of Penicillium aurantiogriseum partiti-like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes - Nerva L., Silvestri A., Ciuffo M., Palmano S., Varese G.C., Turina M. - Environmental Microbiology , 19: 4480-4492
  • 2016 - Resistance to Tospoviruses in Vegetable Crops: Epidemiological and Molecular Aspects - Turina M., Kormelink R., Resende R.O. - Annual Review Of Phytopathology , 54: 347-371
  • 2016 - Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi - Nerva L., Ciuffo M., Vallino M., Margaria P., Varese G.c., Gnavi G., Turina M. - Virus Research , 219: 22-38
  • 2016 - Investigation on the partial resistance of Cpkk2 knock out strain of Cryphonectria parasitica to Cryphonectria hypovirus 1 infection in presence of Geneticin and Geneticin resistance gene - Turina M., Rossi M., Moretti M. - Virus Research , 219: 58-61
  • 2016 - Identification of Ourmiavirus 30K movement protein amino acid residues involved in symptomatology, viral movement, subcellular localization and tubule formation - Margaria P., Anderson C.t., Turina M., Rosa C. - Molecular Plant Pathology , 17: 1063-1079
  • 2016 - Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants - Kontra L., Csorba T., Tavazza M., Lucioli A., Tavazza R., Moxon S., Tisza V., Medzihradszky A., Turina M., Burgyan J. - Plos Pathogens , volume 12
  • 2016 - Host-specific accumulation and temperature effects on the generation of dimeric viral RNA species derived from the S-RNA of members of the Tospovirus genus - Bertran A., Ciuffo M., Margaria P., Rosa C., Resende R.O., Turina M. - Journal Of General Virology , 97: 3051-3062
  • 2016 - RNA1-independent replication and GFP expression from tomato marchitez virus isolate M cloned cDNA - Ferriol I., Turina M., Zamora-Macorra E.J. Falk B.W. - Phytopathology , 106: 500-509
  • 2016 - Comparison of small RNA profiles in Nicotiana benthamiana and Solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection - Margaria P., Miozzi L., Ciuffo M., Rosa C., Axtell M.J., Pappu H.R., Turina M. - Virus Research , 211: 38-45
  • 2016 - Molecular identification and biological characterization of a new potyvirus in lettuce - Ciuffo M., Mammella M., Vallino M., Caciagli P., Turina M. - Archives Of Virology , 161: 2549-2554
  • 2016 - A TaqMan real – Time RT – PCR assay for detection of Groundnut ringspot virus - Urzedo Leao E., Tavella L., Krause-Sakate R., Turina M. - Journal Of Plant Pathology , 98: 347-350
  • 2016 - A new tobamovirus infecting tomato crops in Jordan - Salem N., Mansour A., Ciuffo M., Falk B.W., Turina M. - Archives Of Virology , 161: 503-506
  • 2015 - The first complete genome sequences of two distinct European tomato spotted wilt virus isolates - Margaria P., Miozzi L., Ciuffo M., Pappu H.r., Turina M. - Archives Of Virology , 160: 591-595
  • 2015 - The Importance of the KR-Rich Region of the Coat Protein of Ourmia melon virus for Host Specificity, Tissue Tropism, and Interference With Antiviral Defense. - Rossi M., Vallino M., Abbà S., Ciuffo M., Balestrini R., Genre A., Turina M. - Molecular Plant-Microbe Interactions, 28:30-41
  • 2015 - Evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates - Margaria P., Ciuffo M., Rosa C., Turina M. - Virus Research , 196: 157-161
  • 2015 - Small RNA profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected Nicotiana benthamiana - Margaria P., Miozzi L., Rosa C., Axtell M.j., Pappu H.r., Turina M. - Virus Research , 208: 30-38
  • 2014 - The NSs protein of Tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis - Margaria P.,Bosco L.,Vallino M.,Ciuffo M.,Mautino G. C.,Tavella L.,Turina M. - Journal Of Virology , 88:5788-5802
  • 2014 - The complete genome sequence of polygonum ringspot virus - Margaria P., Miozzi L., Ciuffo M., Pappu H., Turina M. - Archives Of Virology , 159:3149-3152
  • 2014 - Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae - Zhai Y.,Bag S.,Mitter N.,Turina M.,Pappu H.R. - Archives Of Virology , 159:1499-1504
  • 2014 - Molecular characterization of Tomato Spotted Wult Virus in Montenegro - Zindovic J.,Ciuffo M.,Turina M. - Journal Of Plant Pathology , 96:201-205
  • 2014 - Genetic dissection of a putative nucleolar localization signal in the coat protein of ourmia melon virus - Rossi M.,Genre A.,Turina M. - Archives Of Virology , 159:1187-1192
  • 2014 - A new ilarvirus isolated from Viola x wittrockiana and its detection in pansy germoplasm by qRT-PCR - Ciuffo M., Pacifico D., Margaria P., Turina M. - Archives Of Virology , 159:561-565
  • 2014 - Functional characterization of the three mitogen-activated protein kinase kinases (MAP2Ks) present in the Cryphonectria parasitica genome reveals the necessity of Cpkk1 and Cpkk2, but not Cpkk3, for pathogenesis on chestnut (Castanea spp.) - Moretti M.,Rossi M.,Ciuffo M.,Turina M. - Mppol. Molecular Plant Pathology, 15:500-512
  • 2014 - A New Virulent Isolate of Clover Yellow Vein Virus on Phaseolus vulgaris in Bulgaria - Pasev G.,Kostova D.,Turina M. - Journal Of Phytopathology , 162:703-711
  • 2013 - Protocollo diagnostico per Tomato infectious chlorosis virus e Tomato chlorosis virus su pomodoro - Manglli A.,Ciuffo M.,Mascia T.,Davino S.W.,Gallitelli D.,Turina M.,Tomassoli L. - Petria , 23:545-566
  • 2012 - The mycovirus CHV1 disrupts secretion of a developmentally regulated protein in Cryphonectria parasitica - Kazmierczak P., McCabe P., Turina M., Jacob-Wilk D., Van Alfen N.K. - Journal of Virology, 86:6067-6074
  • 2012 - Preliminary evidence of recovery from Tomato spotted wilt virus infection in Frankliniella occidentalis individuals - Mautino G.C., Sacco D., Ciuffo M., Turina M., Tavella L. - Annals of Applied Biology, 161:266-276
  • 2012 - Tospoviruses in the Mediterranean Area - Turina M., Tavella L., Ciuffo M. - Advances in Virus Research, 84:403-437
  • 2012 - Differential expression of the putative Kex2 processed and secreted aspartic proteinase gene family of Cryphonectria parasitica - Jacob-Wilk D., Moretti M., Turina M. Kazmierczak P., Van Alfen N.K. - Fungal Biology , 116:363-368
  • 2011 - Reverse Genetic Analysis of Ourmiaviruses Reveals the Nucleolar Localization of the Coat Protein in Nicotiana benthamiana and Unusual Requirements for Virion Formation - Crivelli G., Ciuffo M., Genre A., Masenga V., Turina M. - Journal Of Virology (62788J0), 85(10): 5091-5104
  • 2011 - Diffusione del virus BlScV sul mirtillo gigante americano - Pansa M.G., Ciuffo M., Gotta P., Frati S., Baudino M., Frontuto A., Prodorutti D., Tavella L., Turina M. - L’informatore Agrario , 34: 76-78
  • 2011 - First Report of Tomato spotted wilt virus on Pepper in Montenegro - J. Zindovic, A. Bulajio, B. Kristic, M. Ciuffo, P. Margaria, M. Turina - Plant Disease (01997J0), 95(7): 882
  • 2011 - Ourmiavirus - Rastgou M., Turina M., Milne R.G. - Virus Taxonomy Ix Reporth , Publisher: Elsevier
  • 2011 - Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke? - Ciuffo M., Testa M., Lenzi R., Turina M. - Archives Of Virology (15471J0), 156(6): 1053-1057
  • 2011 - Molecular characterization of two distinct strains of blueberry scorch virus (BlScV) in northern Italy - Moretti M., Ciuffo M., Gotta P., Prodorutti D., Bragagna P., Turina M. - Archives Of Virology (15471J0), 156(7): 1295-1297
  • 2010 - Cloning of the glyceraldehyde 3-phosphate dehydrogenase gene of Flavescence Dorée Phytoplasma and development of serological and molecular tools for studying its expression. - Margaria P., Palmano S., Turina M. - Journal Of Phytopathology, 158:382-386
  • 2010 - Study of mRNA Expression by Real Time PCR of Cpkk1, Cpkk2 and Cpkk3, three MEKs of Cryphonectria parasitica, in Virus-free and Virus-infected Isogenic Isolates. - Rostagno, L., Crivelli, G., Turina, M. - Journal Of Phytopathology, 158:409-416
  • 2010 - A mutation in the Lettuce infectious yellows virus minor coat protein disrupts whitefly transmission but not in planta systemic movement - Stewart L. R., Medina V., Tian T., Turina M. - Journal Of Virology, 84:12165-12173
  • 2010 - Identification of Dictyothrips betae as the vector of Polygonum ring spot virus - Ciuffo M., Mautino G.C., Bosco l., Turina M., Tavella L. - Annals Of Applied Biology, 157:299-307
  • 2009 - Characterization of Iris Yellow Spot Virus isolates from onion crops in northern Italy - Tomassoli L., Tiberini A., Masenga V., Vicchi V., Turina M. - Journal of Plant Pathology, 91: 733-739
  • 2009 - Agroinoculation of the Crinivirus, Lettuce infectious yellows virus, for systemic plant infection - Wang J., Turina M., Stewart L.R., Lindbo, J.A., Falk, B.W. - Virology, 392:131-136
  • 2009 - Segnalazione nel Parmense del virus PaMV su pomodoro - Vicchi V., Fini P., Gozzi R., Testi V., Delvago C., Turina M. - L'Informatore Agrario , 39:74-75
  • 2009 - Silencing of Kex2 significantly diminishes the virulence of Cryphonectria parasitica - Jacob-WilkD., Turina M,. Kazmierczak P., Van Alfen N.K. - Molecular Plant-Microbe Interactions, 22:211-221
  • 2009 - Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin - Rastgou M. , Habibi M.K., Izadpanah K., Masenga V., Milne R.G., Wolf Y.I., Koonin E.V., Turina M. - Journal of General Virology, 90:2525-2535
  • 2009 - Synergistic interaction between the Potyvirus, Turnip mosaic virus and the Crinivirus, Lettuce infectious yellows virus in plants and protoplasts - Wang J., Turina M., Medina V., Falk B.W. - Virus Research, 144:163-170
  • 2009 - Detection of Flavescence dorée and Bois noir phytoplasmas, Grapevine leafroll associated virus‐1 and ‐3 and Grapevine virus A from the same crude extract by reverse transcription‐RealTime Taqman assays - Margaria P., Turina M., Palmano S. - Plant Pathology, 58:838-845
  • 2008 - The small genomic segment of tomato spotted wilt virus isolate BR20 is necessary but not sufficient to induce lethal necrosis in Nicotiana benthamiana and local necrotic lesions in Nicotiana tabacum cv. white burley - Margaria P., Pacifico D., Ciuffo M., Turina M. - Journal Of Plant Pathology (00077NN), 90: 337-343
  • 2008 - Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate - Galetto, L., Fletcher, J., Bosco, D., Turina, M., Wayadande, A., Marzachi', C. - Canadian Journal Of Microbiology (25960J0), 54(5): 341-351