My PhD is focused on the optimization of molecular tools applied for early detection methods in nursery and forest plants. Target organisms belong to the genus Phytophthora, Oomycetes group. Their pathogenicity is evaluated by PCR and qPCR analysis of sampled material.
Phytophthora economical incidence is a well known problem as worldwide researchers and politicians are usual to the argument. Biological cycles and ecology of several species are well explained, while, especially in UE area, efficiency of bio-security protocols and tools is too low.
Specific variability and pathogen quantification of several environmental samples are estimated by the use of high resolution instruments (Real Time qPCR; Piro Sequencing); Phytophthora pathogenicity grade on host plants has been studied under lab condition.