Mining ectomycorrhizal genomes and transcriptomes: from pan-genome to adaptive evolution in a climate change scenario

The purpose of the project related to Proposal 507866 – CSP New Investigator Proposal (2021 Q3 NI) of DOE Joint Genome Institute is the whole genome re-sequencing of 36 different isolates and fruit bodies belonging to four widespread ectomycorrhizal fungal species, i.e., Tuber borchii, Suillus collinitus, Pisolithus tinctorius, and Scleroderma citrinum, collected in a plethora of habitats different from geographical and microclimatic point of view. The re-sequencing will allow to perform a population genomics and functional gene characterization of these species potentially facing the effect of the temperature increase due to climate change.
A second purpose is the RNA-seq of 6 selected isolates of P. tinctorius and T. borchii during in vitro growth under different temperatures, miming the future expected global warming. The integration of genomic and transcriptomic data will provide useful information on pan-genomes of widespread ectomycorrhizal fungi cohabiting together, as well as their local adaptive evolution at genomic level.

Project leader: Fabiano Sillo
IPSP personnel: Elisa Zampieri, Raffaella Balestrini.
Start: 27/10/2021
End: 27/10/2024